Placeholder Image

Subtitles section Play video

  • Here’s how a spectrophotometer works. A lamp provides the source of light. The beam

  • of light strikes the diffraction grating, which works like a prism and separates the

  • light into its component wavelengths. The grating is rotated so that only a specific

  • wavelength of light reaches the exit slit. Then the light interacts with the sample.

  • From this point, the detector measures the transmittance and absorbance of the sample.

  • Transmittance refers to the amount of light that passes completely through the sample

  • and strikes the detector. Absorbance is a measurement of light that is absorbed by the

  • sample. The detector senses the light being transmitted through the sample and converts

  • this information into a digital display.

Here’s how a spectrophotometer works. A lamp provides the source of light. The beam

Subtitles and vocabulary

Click the word to look it up Click the word to find further inforamtion about it