when Charles Darwin was still a student at Cambridge,

the budding naturalist tore some old bark off a tree

and found two rare beetles underneath.

He'd just taken one beetle in each hand when he spotted a third beetle.

Stashing one of the insects in his mouth for safekeeping,

he reached for the new specimen –

when a sudden spray of hot, bitter fluid scalded his tongue.

Darwin's assailant was the bombardier beetle.

It's one of thousands of animal species,

like frogs,

jellyfish,

salamanders,

and snakes,

that use toxic chemicals to defend themselves –

in this case, by spewing poisonous liquid from glands in its abdomen.

But why doesn't this caustic substance, ejected at 100 degrees Celsius,

hurt the beetle itself?

In fact, how do any toxic animals survive their own secretions?

The answer is that they use one of two basic strategies:

securely storing these compounds

or evolving resistance to them.

Bombardier beetles use the first approach.

They store ingredients for their poison in two separate chambers.

When they're threatened, the valve between the chambers opens

and the substances combine in a violent chemical reaction

that sends a corrosive spray shooting out of the glands,

passing through a hardened chamber that protects the beetle's internal tissues.

Similarly, jellyfish package their venom safely

in harpoon-like structures called nematocysts.

And venomous snakes store their flesh-eating, blood-clotting compounds

in specialized compartments that only have one exit:

through the fangs and into their prey or predator.

Snakes also employ the second strategy: built-in biochemical resistance.

Rattlesnakes and other types of vipers manufacture special proteins

that bind and inactivate venom components in the blood.

Meanwhile, poison dart frogs have also evolved resistance to their own toxins,

but through a different mechanism.

These tiny animals defend themselves using hundreds of bitter-tasting compounds

called alkaloids

that they accumulate from consuming small arthropods like mites and ants.

One of their most potent alkaloids is the chemical epibatidine,

which binds to the same receptors in the brain as nicotine

but is at least ten times stronger.

An amount barely heavier than a grain of sugar would kill you.

So what prevents poison frogs from poisoning themselves?

Think of the molecular target of a neurotoxic alkaloid as a lock,

and the alkaloid itself as the key.

When the toxic key slides into the lock,

it sets off a cascade of chemical and electrical signals

that can cause paralysis,

unconsciousness,

and eventually death.

But if you change the shape of the lock, the key can't fit.

For poison dart frogs and many other animals with neurotoxic defenses,

a few genetic changes alter the structure of the alkaloid-binding site

just enough to keep the neurotoxin from exerting its adverse effects.

Poisonous and venomous animals

aren't the only ones that can develop this resistance:

their predators and prey can, too.

The garter snake, which dines on neurotoxic salamanders,

has evolved resistance to salamander toxins

through some of the same genetic changes as the salamanders themselves.

That means that only the most toxic salamanders can avoid being eaten—

and only the most resistant snakes will survive the meal.

The result is that the genes providing the highest resistance and toxicity

will be passed on in greatest quantities to the next generations.

As toxicity ramps up, resistance does too,

in an evolutionary arms race that plays out over millions of years.

This pattern appears over and over again.

Grasshopper mice resist painful venom from scorpion prey

through genetic changes in their nervous systems.

Horned lizards readily consume harvester ants,

resisting their envenomed sting with specialized blood plasma.

And sea slugs eat jellyfish nematocysts,

prevent their activation with compounds in their mucus,

and repurpose them for their own defenses.

The bombardier beetle is no exception:

the toads that swallow them

can tolerate the caustic spray that Darwin found so distasteful.

Most of the beetles are spit up hours later,

amazingly alive and well.

But how do the toads survive the experience?

That is still a mystery.